FUTURA BIOLABS

Reconstituting &
Storage Guide

Everything you need to know about peptide storage and reconstitution — plus our built-in calculator for precise research measurements.

LIVE DEMO

Reconstitution Process

FUTURA
BIOLABS
RESEARCH USE ONLY

Ready for reconstitution

1

Equalise vial pressure with sterile needle

2

Add 1–2 mL bacteriostatic water slowly

3

Gently swirl — never shake vigorously

4

Store at 2–8°C up to 8 weeks

RESEARCH TOOL

Peptide Calculator

Enter your peptide vial size and the volume of bacteriostatic water you will add. The calculator returns the final concentration per mL.

Enter values above to see your concentration

For in vitro research use only. All calculations are for research planning purposes only.

STORAGE

Preserving Quality & Stability

−20°C

Freezer Storage

Lyophilised peptides remain stable for up to 48 months. Ideal for long-term preservation.

Best for long-term

2–8°C

Fridge Storage

Reconstituted peptides should be stored at 2–8°C and used within 4–8 weeks.

Post-reconstitution

20–25°C

Room Temperature

Short-term only during active use. Lyophilised peptides tolerate brief room temp exposure.

Transit only
Peptide TypeLyophilised StorageReconstituted Storage
Basic PeptidesUp to 48 months at −20°C2–8 weeks at 2–8°C
Acidic PeptidesUp to 48 months at −20°C2–8 weeks at 2–8°C
Neutral PeptidesUp to 48 months at −20°C2–8 weeks at 2–8°C
Hydrophobic PeptidesUp to 48 months at −20°C2–8 weeks at 2–8°C
Hydrophilic PeptidesUp to 48 months at −20°C2–8 weeks at 2–8°C

PROTOCOL

Reconstitution Protocol

Choosing Your Solvent

Bacteriostatic water is the standard solvent for most peptides. Hydrophobic peptides may require a small amount of acetic acid (0.1% AA) to achieve complete dissolution. Always create a stock solution at higher concentration than needed, allowing for later dilution.

Equalising Pressure

Insert a sterile needle half-filled with air into the rubber stopper before adding solvent. The vial will either draw in or release air, stabilising internal pressure and preventing solution loss through the stopper during subsequent handling.

Mixing Technique

Gently swirl — never shake vigorously. Vigorous agitation creates foam and can physically damage the peptide structure through mechanical shear. If particles remain after swirling, gentle sonication can assist complete dissolution.

Avoiding Over-Dilution

Use between 1–2.5 mL of solvent per vial unless specific studies confirm a higher volume is safe. Excessive dilution compromises peptide integrity and reduces shelf life. Use our calculator above to determine optimal concentration.

Filtration

For maximum sterility, filter the reconstituted solution through a 0.2 µm sterile syringe filter before use. This removes any potential microbial contamination introduced during the reconstitution process.

Post-Reconstitution Storage

Once reconstituted, label the vial with the date and concentration. Store at 2–8°C in a refrigerator. Use within 4–8 weeks. Minimise freeze-thaw cycles — aliquot into single-use vials if the full volume will not be used within a session.

FOR RESEARCH USE ONLY

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